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US 11,970,691 B2Granted
Immobilized thermostable trehalose synthase and method for producing trehalose and trehalulose by using same
AssigneeNational Taiwan Normal University
InventorGuan-Chiun Lee
Life of this patent
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Abstract
Provided is an immobilized thermostable trehalose synthase including an amino acid sequence of a trehalose synthase domain and an amino acid sequence of a cellulose binding domain. Also provided is a method for converting maltose into trehalose or for converting sucrose into trehalulose by using the immobilized thermostable trehalose synthase.
Claims15
A thermostable trehalose synthase, consisting essentially of:
- an amino acid sequence of a trehalose synthase domain derived from Thermus thermophilus;
- wherein said trehalose synthase domain converts maltose into trehalose and/or converts sucrose into trehalulose;
- and
- an amino acid sequence of a cellulose binding domain;
- wherein said amino acid sequence of said cellulose binding domain has at least 90% sequence identity to SEQ ID NO:
- 2, and wherein said cellulose binding domain binds cellulose;
- wherein said amino acid sequence of said cellulose binding domain is connected to a C-terminal of said amino acid sequence of said trehalose synthase domain through a linker, and said linker consists of consecutive amino acids selected from Threonine and Proline at an N-terminal of SEQ ID NO:
- 2.
5 more elements
Dependencies are inferred from the claim text.