Method for producing an Entada seed extract enriched with metabolites of interest, extract produced by such a method, and cosmetic and dermocosmetic applications of such an extract
AssigneeExsymol
InventorMarie-Christine Seguin
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Abstract
The invention relates to a method for producing an extract of seeds of the Entada genus by selective hydrolysis of the metabolites accumulated in the seeds. The invention also relates to the extract produced using such a method, to a composition comprising such an extract, and to the cosmetic and dermocosmetic applications of such an extract.
Claims28
A process for producing an extract of seeds of the genus Entada enriched in entadamide A, comprising:
- (i) activating endogenous enzyme(s) by dispersion of the seeds under mechanical stirring in a seed/water weight ratio of about 0.02 to about 2;
- (ii) enzymatically hydrolyzing the dispersion obtained in step (i) by heat treatment at a temperature between about 25 and about 100° C. and for a time period of between about 2 minutes and about 12 hours, at a pH of between about 4 and about 8;
- (iii) inhibiting the enzymatic activity in the dispersion obtained in step (ii), the inhibition of said enzymatic activity comprising:
- a) adding an agent irreversibly inhibiting the activity of the endogenous enzymes in a volume ratio of inhibiting agent/water of between about 0.1 and about 10;
- b) heating the reaction medium obtained in step (iii/a) at a temperature of between about 25 and about 75° C. and for a time period of between about 2 minutes and about 12 hours;
- c) removing the inhibiting agent under reduced pressure to obtain an aqueous filtrate enriched in entadamide A and containing a native non-hydrolyzed phaseoloidin;
- (iv) adding a cosmetically acceptable solvent to the aqueous filtrate obtained in step (iii/c), then adjusting the pH between about 3 and about 7.
A composition which comprises a seed extract of the genus Entada enriched in entadamide A and one or more additives which are physiologically compatible with skin or appendages, wherein the amount of said extract is between 1% and 10% by weight relative to the total weight of the composition, wherein the seed extract is obtained by a process comprising:
- (i) activating endogenous enzyme(s) by dispersion of seeds of the genus Entada under mechanical stirring in a seed/water weight ratio of about 0.02 to about 2;
- (ii) enzymatically hydrolyzing the dispersion obtained in step (i) by heat treatment at a temperature between about 25 and about 100° C. and for a time period of between about 2 minutes and about 12 hours, at a pH of between about 4 and about 8;
- (iii) inhibiting the enzymatic activity in the dispersion obtained in step (ii), the inhibition of said enzymatic activity comprising:
- a) adding an agent irreversibly inhibiting the activity of the endogenous enzymes in a volume ratio of inhibiting agent/water of between about 0.1 and about 10;
- b) heating the reaction medium obtained in step (iii/a) at a temperature of between about 25 and about 75° C. and for a time period of between about 2 minutes and about 12 hours;
- c) removing the inhibiting agent under reduced pressure to obtain an aqueous filtrate enriched in entadamide A and containing a native non-hydrolyzed phaseoloidin,
- (iv) adding a cosmetically acceptable solvent to the aqueous filtrate obtained in step (iii/c), then adjusting the pH between about 3 and about 7, wherein the seed extract comprises a weight content of between about 1 and about 5 mg/g of entadamide A, a weight content of between about 9 and about 85 mg/g of phaseoloidin, and traces of entadamide A glucone not exceeding a weight content of 0.2 mg/g.
Dependencies are inferred from the claim text.