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US 11,969,246 B2Granted
Method of producing thin enzyme-based sensing layers on planar sensors
AssigneeCalifornia Institute of Technology
InventorsSamson Chen, Axel Scherer, Dvin I. Adalian, Peter Petillo, Muhammad Musab Jilani, Xiomara L. Madero, Deepan Kishore Kumar
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Abstract
A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.
Claims18
A method, comprising:
- spin coating an aqueous solution of a signal transduction enzyme onto a device having a plurality of electrodes, the signal transduction enzyme being configured to detect an active compound capable of generating an electric current;
- at least partially drying the spin coated signal transduction enzyme and crosslinking the signal transduction enzyme with a crosslinker within a temperature-controlled chamber to produce a cross-linked enzyme film having a thickness of less than 1 micrometer, and
- wherein the signal transduction enzyme is configured so that a current measured by the plurality of electrodes settles within about 5% of its stable value within 1 second.
A method, comprising:
- spin coating a solution of a signal transduction enzyme onto a device having a plurality of electrodes, the signal transduction enzyme being configured to detect an active compound capable of generating an electric current;
- at least partially drying the spin coated signal transduction enzyme and crosslinking the signal transduction enzyme by heating the spin coated signal transduction enzyme in the presence of a crosslinker, to produce a cross-linked enzyme film having a thickness of less than 5 micrometers, and
- wherein the signal transduction enzyme is configured so that a current measured by the plurality of electrodes settles within about 5% of its stable value within 1 second.
Dependencies are inferred from the claim text.