Arginine supplementation to improve efficiency in gas fermenting acetogens
AssigneeLanzaTech NZ, Inc.
InventorsKaspar Valgepea, Michael Koepke, James Bruce Yarnton Haycock Behrendorff, Esteban Marcellin, Lars K. Nielsen, Renato de S. P. Lemgruber
Life of this patent
Estimating the term…
Abstract
The invention provides methods for improving efficiency of fermentation by arginine supplementation, and genetically modified bacterium for use therefor. More particularly the invention provides methods for (i) increasing the production ATP intensive products with arginine supplementation, (ii) increasing utilization of arginine by a C1-fixing bacterium; and (iii) providing C1-fixing bacterium with optimized arginine de-aminase pathways.
Claims6
A method for improving efficiency of arginine co-utilization with one or more gaseous substrates selected from the group consisting of CO, H2 and CO2, the method comprising culturing a genetically engineered C1-fixing bacterium comprising one or more genetic modifications selected from the group consisting of:
- i) a disruptive mutation in an arginine transporter;
- ii) overexpression of one or more endogenous enzymes selected from the group consisting of arginine deiminase (EC 3.5.3.6), ornithine carbomyltransferase (putrescine carbomyltransferase) (EC 2.1.3.3), carbamate kinase (EC 2.7.2.2), ornithine racemase (EC 5.1.1.12), ornithine aminomutase (EC 5.4.3.5), and 2,4-diaminopentanoate dehydrogenase (EC 1.4.1.12);
- iii) expression of one or more mutated endogenous enzymes selected from the group consisting of arginine deiminase (EC 3.5.3.6), ornithine carbomyltransferase (putrescine carbomyltransferase) (EC 2.1.3.3), carbamate kinase (EC 2.7.2.2), ornithine racemase (EC 5.1.1.12), ornithine aminomutase (EC 5.4.3.5), and 2,4-diaminopentanoate dehydrogenase (EC 1.4.1.12);
- and
- iv) expression of one or more exogenous enzymes selected from the group consisting of arginine deiminase (EC 3.5.3.6), ornithine carbomyltransferase (putrescine carbomyltransferase) (EC 2.1.3.3), carbamate kinase (EC 2.7.2.2), ornithine racemase (EC 5.1.1.12), ornithine aminomutase (EC 5.4.3.5), and 2,4-diaminopentanoate dehydrogenase (EC 1.4.1.12) wherein arginine is a sole nitrogen source.
Dependencies are inferred from the claim text.