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US 11,965,191 B2Granted
Programmable protein circuits in living cells
AssigneeCalifornia Institute of Technology
InventorsXiaojing Gao, Lucy S. Chong, Michael Elowitz, Mark William Budde, Matthew Sun-min Kim
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Abstract
The present disclosure relates to systems, methods and compositions provided herein that include a compound protease. The compound protease can contain a protease domain, a cut site for another enzyme and an association domain. In some embodiments, the compound protease is part of a protein circuit.
Claims20
A synthetic protein circuit, comprising:
- a first protease, optionally comprising an association domain of the first protease;
- a second protease, optionally comprising a complementary association domain of the second protease;
- and
- a first target protein comprising a first degron of the first target protein that destabilizes the first target protein when present on the first target protein by enhancing degradation of the first target protein;
- wherein the first target protein is configured to interact with the first protease, the second protease, a third protease and/or a fourth protease to form an OR, AND, NAND, IMPLY, NIMPLY, XOR or XNOR logic gate.
2 more elements
Dependencies are inferred from the claim text.