Cellobiohydrolase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsMark Wogulis, Leslie DeMars, David Osborn
Life of this patent
Estimating the term…
Abstract
The present invention relates to cellobiohydrolase variants having increased thermal activity or thermostability, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims45
A cellobiohydrolase variant, comprising a substitution relative to SEQ ID NO:
- 1 at one or more of positions 113, 157, 199, 240, 250, 274, 318, 325, 328, 347, 349, 358, 360, 391, 393, 394, 412, and 430 of SEQ ID NO:
- 1, wherein the variant has:
- (1) cellobiohydrolase activity, (2) at least 90%, but less than 100%, sequence identity to SEQ ID NO:
- 1, and (3) an increased thermal activity or increased thermostability relative to the cellobiohydrolase of SEQ ID NO:
- 1.
A method for obtaining a cellobiohydrolase variant, comprising introducing into a parent cellobiohydrolase a substitution at one or more positions corresponding to positions 113, 157, 159, 199, 250, 274, 318, 325, 328, 347, 349, 358, 360, 380, 391, 393, 394, 412, and 430 of the polypeptide of SEQ ID NO:
- 1, wherein the variant has:
- (1) cellobiohydrolase activity, (2) at least 90%, but less than 100%, sequence identity to SEQ ID NO:
- 1, and (3) an increased thermal activity or increased thermostability relative to the cellobiohydrolase of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.