Plasmid system
AssigneeAutolus Limited
InventorsMartin Pulé, Paulo Fernandes, Hanna Kymalainen, Ekaterini Kotsopoulou
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Abstract
There is provided a plasmid system for transfection into a cell to create a producer cell, the system comprising: a. a helper plasmid comprising a first nucleotide sequence encoding Murine leukemia virus (MLV)-derived Gag and Pol poly-proteins; b. an envelope plasmid comprising a second nucleotide sequence encoding an Env protein; c. a genome plasmid comprising a third nucleotide sequence encoding a retroviral genome, wherein the first nucleotide sequence is codon-shuffled to remove any significant regions of homology with the third nucleotide sequence; and wherein the second nucleotide sequence is codon-optimised for expression in the producer cell.
Claims9
A plasmid system for transfection into a cell to create a producer cell, the system comprising:
- a) a helper plasmid comprising a first nucleotide sequence encoding Murine leukemia virus (MLV)-derived Gag and Pol poly-proteins,
- b) an envelope plasmid comprising a second nucleotide sequence encoding an Env protein,
- c) a genome plasmid comprising a third nucleotide sequence comprising a retroviral genome,
- wherein the first nucleotide sequence comprises the sequence selected from SEQ ID NO:
- 1, SEQ ID NO:
- 2 or SEQ ID NO:
- 3, and
- wherein the second nucleotide sequence is codon-optimized for expression in the producer cell.
A nucleotide sequence encoding MLV-derived Gag and Pol poly-proteins comprising the sequence selected from:
- SEQ ID NO:
- 1 to SEQ ID NO:
- 3.
A nucleotide sequence encoding Env protein comprising the sequence selected from:
- SEQ ID NO:
- 5 to SEQ ID NO:
- 7 and SEQ ID NO:
- 9 to 11.
Dependencies are inferred from the claim text.