Back to results
US 11,965,154 B2Granted
Detection of nuclease edited sequences in automated modules and instruments
AssigneeInscripta, Inc.
InventorsEileen Spindler, Isaac Wagner, Clint Davis, Julia Swavola, Phillip Belgrader
Life of this patent
Estimating the term…
Abstract
The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.
Claims17
A method for performing enrichment of cells edited by a nucleic acid-guided nuclease comprising:
- (a) providing transformed cells in growth medium, wherein the cells comprise a coding sequence for a nucleic acid-guided nuclease, a sequence for a donor DNA, and a coding sequence for a gRNA, wherein at least the coding sequence for the gRNA is under the control of an inducible promoter;
- (b) allowing the transformed cells to grow until the cells are in stationary phase;
- (c) inducing transcription of the coding sequence for the gRNA;
- and
- (d) allowing the cells to edit, wherein an editing efficiency is improved compared to inducing transcription of the coding sequence for the gRNA when the transformed cells are in log phase.
2 more elements
A method for performing enrichment of cells edited by a nucleic acid-guided nuclease comprising:
- (a) providing transformed cells in growth medium, wherein the cells comprise a first round of nucleic acid-guided editing components, wherein the first round of nucleic acid-guided editing components comprises a first gRNA under the control of a first inducible promoter;
- (b) allowing the transformed cells to grow until the cells are in stationary phase;
- (c) inducing transcription of the first gRNA;
- (d) allowing the cells to edit, wherein an editing efficiency is improved compared to inducing transcription of the first gRNA when the transformed cells are in log phase;
- and
- (e) transforming the cells with a second round of nucleic acid-guided editing components, wherein the second round of nucleic acid-guided editing components comprises a second gRNA under the control of a second inducible promoter.
3 more elements
Dependencies are inferred from the claim text.