Single-injection methods and formulations to induce and control multiple ovarian follicles in bovine, caprine, ovine, camelid and other female animals

An injectable drug formulation for inducing ovarian follicular hyperstimulation and superovulation in bovine animals, the formulation comprising:

• an in-situ depot formulation comprising a protein microsphere matrix having a diameter ranging from 50-100 microns to a bovine animal, the protein microsphere matrix including controlled release agents, the controlled release agents including a single dose of follicle stimulating hormone (FSH) released over 96 hours beginning 48 hours after administration, and two doses of luteinizing hormone (LH), wherein the first dose of LH provides an immediate surge of LH and the second dose provides at least one calculated LH release (burst) after 144 hours from administration of the protein microsphere matrix,
• wherein the drug formulation is configured to be administered in a single administration of said protein microsphere matrix to a bovine animal, thereby inducing ovarian follicular hyperstimulation and superovulation of the bovine animal when administered to the bovine animal,
• wherein the protein microsphere matrix comprises at least one polymer, wherein the at least one polymer is selected from a group consisting of polyethylene glycol (PEG), poly(lactic acid) (PLA) or poly(lactide-co-glycolide) (PLGA).