Back to results
US 11,963,530 B2Granted
Agricultural compositions comprising remodeled nitrogen fixing microbes
AssigneePivot Bio, Inc.
InventorsMark Reisinger, Ernest Sanders, Richard Broglie, Karsten Temme
Life of this patent
Estimating the term…
Abstract
The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
Claims57
A seed treatment composition, comprising:
- a. a plurality of non-intergeneric remodeled bacteria that have an average colonization ability per unit of plant root tissue of at least about 1.0×107 bacterial cells per gram of fresh weight of plant root tissue and produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour;
- and
- b. at least one pesticide;
- wherein said pesticide is selected from the group consisting of fludioxonil, metalaxyl, mefenoxam, azoxystrobin, thiabendazole, ipconazole, tebuconazole, prothioconazole, imidacloprid, clothianidin, thiamethoxam, chlorantraniliprole, Bacillus pumilus, Bacillus subtilis, captan, hydrogen peroxide, mancozeb, pyraclostrobin, Streptomyces griseoviridis, Streptomyces lydicus, thiram, Trichoderma harzianum Rifai, trifloxystrobin, chlorpyrifos, abamectin, Bacillus firmus, harpin alpha beta protein, indole butyric acid, carboxin, permethrin, triticonazole, ethaboxam, carboxyanilide, broflanilide, and combinations thereof;
- wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a member selected from the group consisting of:
- nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, draT, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof;
- and wherein the plurality of non-intergeneric remodeled bacteria comprise non-intergeneric remodeled bacteria selected from the group consisting of:
- Rahnella aquatilis, Klebsiella variicola, Achromobacter spiritinus, Achromobacter marplatensis, Microbacterium murale, Kluyvera intermedia, Kosakonia pseudosacchari, Enterobacter sp., Azospirillum lipoferum, Kosakonia sacchari, and combinations thereof.
5 more elements
A corn seed treatment composition, comprising:
- a. a plurality of non-intergeneric remodeled bacteria that have an average colonization ability per unit of plant root tissue of at least about 1.0×107 bacterial cells per gram of fresh weight of plant root tissue and produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour;
- and
- b. at least one pesticide selected from the group consisting of:
- clothianidin, thiamethoxam, chlorantraniliprole, imidacloprid, fludioxonil, mefenoxam, azoxystrobin, thiabendazole, metalaxyl, ipconazole, tebuconazole, prothioconazole, and combinations thereof;
- wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a member selected from the group consisting of:
- nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, draT, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, and combinations thereof.
3 more elements
A corn seed treatment composition, comprising:
- a. a plurality of non-intergeneric remodeled bacteria that have an average colonization ability per unit of plant root tissue of at least about 1.0×107 bacterial cells per gram of fresh weight of plant root tissue and produce fixed N of at least about 1×10−17 mmol N per bacterial cell per hour;
- and
- b. at least one pesticide selected from the group consisting of:
- clothianidin, thiamethoxam, chlorantraniliprole, imidacloprid, fludioxonil, mefenoxam, azoxystrobin, thiabendazole, metalaxyl, ipconazole, tebuconazole, prothioconazole, and combinations thereof;
- wherein the plurality of non-intergeneric remodeled bacteria comprise non-intergeneric remodeled bacteria selected from the group consisting of:
- Rahnella aquatilis, Klebsiella variicola, Achromobacter spiritinus, Achromobacter marplatensis, Microbacterium murale, Kluyvera intermedia, Kosakonia pseudosacchari, Enterobacter sp., Azospirillum lipoferum, Kosakonia sacchari, and combinations thereof.
3 more elements
Dependencies are inferred from the claim text.