Multi-modal microscopic imaging
AssigneeInscopix, Inc.
InventorsKoen Visscher, Pei Sabrina Xu, Shay Neufeld
Life of this patent
Estimating the term…
Abstract
An adapter configured to be optically coupled to a plurality of microscopes. The adapter includes a) a first microscope interface configured to optically couple a first microscope to an optical element in optical communication with an optical probe; b) a second microscope interface configured to optically couple a second microscope to the optical element in optical communication with the optical probe; and c) an optical arrangement configured to direct light collected from a sample with aid of the optical probe to (1) the first microscope and second microscope simultaneously, or (2) the first microscope or second microscope selectively.
Claims52
A method for providing multi-modal imaging using a plurality of microscopes, said method comprising:
- a) receiving functional imaging data and structural imaging data from an adapter, the adapter comprising (i) a first microscope interface configured to optically couple a first microscope to an optical element that is in optical communication with an optical probe to provide the functional imaging data of a sample, and (ii) a second microscope interface configured to optically couple a second microscope to the optical element to provide the structural imaging data of the sample;
- b) processing the functional imaging data, received from the adapter, to generate a first overlaid image;
- c) processing the structural imaging data, received from the adapter, to generate a second overlaid image;
- d) co-registering the first overlaid image and the second overlaid image,
- wherein said co-registering comprises aligning at least a portion of the first overlaid image and the second overlaid image based at least in part on a coordinate transformation,
- wherein the coordinate transformation is derived based at least in part on a difference in sizes, shapes, positions, or orientations of a landmark feature present both in the first overlaid image and the second overlaid image;
- e) using the difference in sizes, shapes, positions, or orientations the landmark feature in the first overlaid image and that in the second overlaid image to characterize an amount of distortion or warping of the landmark feature between the first overlaid image and the second overlaid image;
- and
- f) generating a third overlay overlaid image from the first overlaid image and the second overlaid image after said co-registering.
A system configured to effectuate multi-modal imaging, the system comprising:
- an adapter comprising (i) a first microscope interface configured to optically couple a first microscope to an optical element that is in optical communication with an optical probe to provide functional imaging data of a sample, and (ii) a second microscope interface configured to optically couple a second microscope to the optical element to provide structural imaging data of the sample;
- and
- a processor configured
- (i) to process the functional imaging data, received from the adapter, to generate a first overlaid image,
- (ii) to process the structural imaging data, received from the adapter, to generate a second overlaid image,
- (iii) to co-register the first overlaid image and the second overlaid image with the use of aligning at least a portion of the first overlaid image and the second overlaid image based at least in part on a coordinate transformation,
- wherein the coordinate transformation is derived based at least in part on a difference in sizes, shapes, positions, or orientations of a landmark feature present both in the first overlaid image and the second overlaid image;
- (iv) to use the difference in sizes, shapes, positions, or orientations of the landmark feature in the first overlaid image and in the second overlaid image to characterize an amount of distortion or warping of the landmark feature between the first overlaid image and the second overlaid image;
- and
- (v) to generate a third overlaid image from the first overlaid image and the second overlaid image after said co-registering.
Dependencies are inferred from the claim text.