Nanopore sensing with a fluidic passage
AssigneePresident and Fellows of Harvard College
InventorPing Xie
Life of this patent
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Abstract
In a method for sensing translocation of a molecule through a nanopore, a molecule in a first fluidic solution in a first fluidic reservoir that is in direct fluidic connection with the nanopore is directed to a nanopore inlet and translocated through the nanopore to a nanopore outlet and through a fluidic passage that is in direct fluidic connection with the nanopore outlet, to a second fluidic solution in a second fluidic reservoir disposed in direct fluidic connection with the fluidic passage. The fluidic passage has at least one fluidic section in which a length of the fluidic section is greater than a width of the fluidic section. Translocation of the molecule through the nanopore is sensed by measuring the electrical potential local to the fluidic passage during the translocation of the molecule.
Claims37
A method for sensing translocation of a molecule through a nanopore comprising:
- directing to an inlet of the nanopore at least one molecule in a first fluidic solution in a first fluidic reservoir, said first fluidic reservoir being disposed in fluidic connection with the inlet of the nanopore;
- translocating the molecule in the first fluidic solution through the nanopore, out of an outlet of the nanopore, and then through a fluidic passage disposed in fluidic connection with an outlet of the nanopore, said fluidic passage including a passage length having a fluidic passage fluidic resistance, RFP, of at least about 10% of a nanopore fluidic resistance, RPore, of the nanopore, and no more than about 10 times the nanopore fluidic resistance, RPore, and having a passage width that is greater than a diameter of the nanopore and that is less than said passage length;
- directing the molecule to a second fluidic solution in a second fluidic reservoir disposed in fluidic connection with the fluidic passage;
- and
- sensing an electrical potential local to the fluidic passage during translocation of the molecule through the nanopore, to sense translocation of the molecule.
A method for sensing translocation of a molecule through a nanopore comprising:
- directing to an inlet of the nanopore at least one molecule in a first fluidic solution in a first fluidic reservoir, said first fluidic reservoir being disposed in fluidic connection with the inlet of the nanopore;
- translocating at least a section of the molecule in the first fluidic solution through the nanopore and then into a fluidic passage disposed in fluidic connection with the nanopore, said fluidic passage including a passage length having a fluidic passage fluidic resistance, RFP, of at least about 10% of a nanopore fluidic resistance, RPore, of the nanopore, and no more than about 10 times the nanopore fluidic resistance, RPore, and having a passage width that is greater than a diameter of the nanopore and that is less than said passage length;
- and
- sensing an electrical potential local to the fluidic passage during translocation of said at least a section of the molecule through the nanopore, to sense translocation of said at least a section of the molecule.
A method for sensing translocation of a molecule through a nanopore comprising:
- directing to an inlet of the nanopore at least one molecule in a first fluidic solution in a first fluidic reservoir, said first fluidic reservoir being disposed in fluidic connection with the inlet of the nanopore;
- translocating the molecule in the first fluidic solution through the nanopore, out of an outlet of the nanopore, and then through a fluidic passage disposed in fluidic connection with an outlet of the nanopore, said fluidic passage including a passage length having a fluidic passage fluidic resistance, RFP, of at least about 10% of a nanopore fluidic resistance, RPore, of the nanopore, and no more than about 10 times the nanopore fluidic resistance, RPore, and having a passage width that is greater than a diameter of the nanopore and that is less than said passage length;
- and
- sensing an electrical potential local to the fluidic passage during translocation of the molecule through the nanopore, to sense translocation of the molecule.
Dependencies are inferred from the claim text.