Method for measuring amount of blood component in blood
AssigneePhc Holdings Corporation
InventorsMasaki Fujiwara, Yoshifumi Takahara, Takaaki Fujii, Setsuko Yano, Fuminori Kutsuna
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Abstract
The present invention provides a method for accurately measuring a blood component despite uneven distribution of blood introduced into a capillary. The measurement method according to the present invention is characterized in that a plurality of electrode systems for measuring the hematocrit are provided in a capillary of a biosensor to measure the hematocrit at different positions in the capillary. By measuring the hematocrit at the plurality of positions in the capillary as described above, the hematocrit can be measured mom accurately despite uneven distribution of blood introduced into the capillary.
Claims14
A method for measuring the amount of a blood component in blood using a biosensor,
- the biosensor comprising:
- a first electrode system for measuring a blood component amount-dependent current value, the first electrode system including a first working electrode and a first counter electrode;
- a second electrode system for measuring a blood component amount-dependent current value, the second electrode system including a second working electrode and a second counter electrode;
- and
- a third electrode system for measuring a hematocrit-dependent current value, the third electrode system including a third working electrode and a third counter electrode,
- wherein a first reagent layer is disposed on the first electrode system,
- a second reagent layer is disposed on the second electrode system,
- the first reagent layer and the second reagent layer are disposed spaced apart from each other,
- the first reagent layer and the second reagent layer each contain a reagent for measuring the amount of the blood component in the blood,
- at least one of the first counter electrode, the second working electrode, and the second counter electrode is used as the third working electrode, and
- at least one of the first working electrode, the first counter electrode, and the second counter electrode is used as the third counter electrode,
- the method comprising:
- a first step of applying a first voltage to the first electrode system and detecting a first blood component amount-dependent current value;
- a second step of applying a second voltage to the second electrode system and detecting a second blood component amount-dependent current value;
- a third step of applying a third voltage to the third electrode system and detecting a third hematocrit-dependent current value;
- and
- a step of calculating the amount of the blood component using the first blood component amount-dependent current value, the second blood component amount-dependent current value, and the third hematocrit-dependent current value,
- wherein all portions of the first step and the second step are simultaneously performed,
- the first step is performed at least twice,
- the second step is performed at least twice, and
- two or more first blood component amount-dependent current values obtained are used as the first blood component amount-dependent current values in the step of calculating the amount of the blood component.
A method for measuring the amount of a blood component in blood using a biosensor,
- the biosensor comprising:
- a first electrode system for measuring a blood component amount-dependent current value, the first electrode system including a first working electrode and a first counter electrode;
- a second electrode system for measuring a blood component amount-dependent current value, the second electrode system including a second working electrode and a second counter electrode;
- and
- a third electrode system for measuring a hematocrit-dependent current value, the third electrode system including a third working electrode and a third counter electrode,
- wherein a first reagent layer is disposed on the first electrode system,
- a second reagent layer is disposed on the second electrode system,
- the first reagent layer and the second reagent layer are disposed spaced apart from each other,
- the first reagent layer and the second reagent layer each contain a reagent for measuring the amount of the blood component in the blood,
- at least one of the first counter electrode, the second working electrode, and the second counter electrode is used as the third working electrode, and
- at least one of the first working electrode, the first counter electrode, and the second counter electrode is used as the third counter electrode,
- the method comprising:
- a first step of applying a first voltage to the first electrode system and detecting a first blood component amount-dependent current value;
- a second step of applying a second voltage to the second electrode system and detecting a second blood component amount-dependent current value;
- a third step of applying a third voltage to the third electrode system and detecting a third hematocrit-dependent current value;
- and
- a step of calculating the amount of the blood component using the first blood component amount-dependent current value, the second blood component amount-dependent current value, and the third hematocrit-dependent current value,
- wherein the first step is performed at least twice,
- the second step is performed at least twice,
- portions of the first steps and the second steps are simultaneously performed, and
- two or more first blood component amount-dependent current values obtained are used as the first blood component amount-dependent current values in the step of calculating the amount of the blood component.
A method for measuring the amount of a blood component in blood using a biosensor,
- the biosensor comprising:
- a first electrode system for measuring a blood component amount-dependent current value, the first electrode system including a first working electrode and a first counter electrode;
- a second electrode system for measuring a blood component amount-dependent current value, the second electrode system including a second working electrode and a second counter electrode;
- and
- a third electrode system for measuring a hematocrit-dependent current value, the third electrode system including a third working electrode and a third counter electrode,
- a fourth electrode system for measuring a hematocrit-dependent current value, the fourth electrode system including a fourth working electrode and a fourth counter electrode,
- wherein at least one of the first counter electrode, the second working electrode, and the second counter electrode is used as the third working electrode,
- at least one of the first working electrode, the first counter electrode, and the second counter electrode is used as the third counter electrode,
- the first counter electrode is used as the fourth working electrode,
- the second counter electrode is used as the fourth counter electrode,
- a first reagent layer is disposed on the first electrode system,
- a second reagent layer is disposed on the second electrode system,
- the first reagent layer and the second reagent layer are disposed spaced apart from each other,
- the first reagent layer and the second reagent layer each contain a reagent for measuring the amount of the blood component in the blood,
- the method comprising:
- a first step of applying a first voltage to the first electrode system and detecting a first blood component amount-dependent current value;
- a second step of applying a second voltage to the second electrode system and detecting a second blood component amount-dependent current value;
- a third step of applying a third voltage to the third electrode system and detecting a third hematocrit-dependent current value;
- the method further includes a fourth step of applying a fourth voltage to the fourth electrode system and detecting a fourth hematocrit-dependent current value;
- and
- a step of calculating the amount of the blood component,
- wherein the first step is performed at least twice,
- two or more first blood component amount-dependent current values obtained are used as the first blood component amount-dependent current values in the step of calculating the amount of the blood component, and
- the step of calculating the amount of the blood component includes a step of calculating the amount of the blood component using the first blood component amount-dependent current value, the second blood component amount-dependent current value, the third hematocrit-dependent current value, and the fourth hematocrit-dependent current value.
Dependencies are inferred from the claim text.