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US 11,959,836 B2Granted
Analysis system and methods of use thereof
AssigneeGeorgia Tech Research Corporation
InventorsAndrei G. Fedorov, Mason Chilmonczyk, Peter Arthur Kottke
Life of this patent
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Abstract
The present disclosure provides for analysis systems that are configured to extract a fluid sample from a fluid (e.g., aqueous solution) in a reactor (e.g., bioreactor) at a first rate and then flow the fluid sample to a sensor system at a second rate to analyze the fluid sample. The sensor system can detect the presence and/or concentration of molecules (e.g., biomolecules such as biomarkers (e.g., metabolites, proteins, peptides, cytokines, growth factors, DNA, RNA, lipids) and cells of different types and cell properties, e.g., mechanical stiffness, etc.)). The data obtained can be used by a feedback control system to modify, as needed, the conditions in the reactor to enhance the productively of the reactor.
Claims19
A system comprising:
- a flow system including a pump system and a valve system, wherein the pump system and the valve system are in fluidic communication;
- a sampling system in fluidic communication with the flow system;
- a reactor comprising a fluid, wherein the sampling system is in fluidic communication with the reactor, wherein in a first configuration of the flow system, the fluid sample is flowed from the reactor to the pump system through the valve system at a first flow rate;
- and
- a sensor system in fluidic communication with the flow system, wherein in a second configuration of the valve system the fluid sample is flowed from the pump system to the sensor system at a second flow rate, wherein the sensor system is configured to analyze the fluid sample,
- wherein the sample system comprises an extraction element that is dimensionally configured to extract the fluid sample comprising secretome, metabolome, transcriptome, genome, lipidome, or combinations thereof and excluding an intact cell, a microcarrier including one or more intact cells, or combinations thereof.
3 more elements
A system comprising:
- a flow system including a pump system and a valve system, wherein the pump system and the valve system are in fluidic communication;
- a sampling system in fluidic communication with the flow system;
- a reactor comprising a fluid, wherein the sampling system is in fluidic communication with the reactor, wherein in a first configuration of the flow system the fluid sample is flowed from the reactor to the pump system through the valve system at a first flow rate;
- and
- a sensor system in fluidic communication with the flow system, wherein in a second configuration of the valve system the fluid sample is flowed from the pump system to the sensor system at a second flow rate, wherein the sensor system is configured to analyze the fluid sample wherein the sampling system comprises an extraction element that is dimensionally configured to extract the fluid sample comprising a microcarrier including one or more intact cells, wherein the extraction element is further dimensionally configured to trap the microcarrier.
2 more elements
A system comprising:
- a flow system including a pump system and a valve system, wherein the pump system and the valve system are in fluidic communication;
- a sampling system in fluidic communication with the flow system;
- a reactor comprising a fluid, wherein the sampling system is in fluidic communication with the reactor, wherein in a first configuration of the flow system the fluid sample is flowed from the reactor to the pump system through the valve system at a first flow rate;
- and
- a sensor system in fluidic communication with the flow system, wherein in a second configuration of the valve system the fluid sample is flowed from the pump system to the sensor system at a second flow rate, wherein the sensor system is configured to analyze the fluid sample, further comprising a separation/fractionation/trapping system in fluidic communication with the flow system and the sampling system, wherein the separation/fractionation/trapping system is configured to separate a first group of components from the fluid sample to produce a separated/fractionated fluid sample, wherein the separated/fractionated fluid sample is analyzed by the sensor system.
2 more elements
A system comprising:
- a flow system including a pump system and a valve system, wherein the pump system and the valve system are in fluidic communication;
- a sampling system in fluidic communication with the flow system;
- a reactor comprising a fluid, wherein the sampling system is in fluidic communication with the reactor, wherein in a first configuration of the flow system the fluid sample is flowed from the reactor to the pump system through the valve system at a first flow rate;
- and
- a sensor system in fluidic communication with the flow system, wherein in a second configuration of the valve system the fluid sample is flowed from the pump system to the sensor system at a second flow rate, wherein the sensor system is configured to analyze the fluid sample, further comprising a separation system in fluidic communication with the flow system and the sensor system, wherein the separation system is configured to separate a first group of components from the fluid sample to produce a separated fluid sample, wherein the separated fluid sample is analyzed by the sensor system.
2 more elements
A system comprising:
- a flow system including a pump system and a valve system, wherein the pump system and the valve system are in fluidic communication;
- a sampling system in fluidic communication with the flow system;
- a reactor comprising a fluid, wherein the sampling system is in fluidic communication with the reactor, wherein in a first configuration of the flow system the fluid sample is flowed from the reactor to the pump system through the valve system at a first flow rate;
- and
- a sensor system in fluidic communication with the flow system, wherein in a second configuration of the valve system the fluid sample is flowed from the pump system to the sensor system at a second flow rate, wherein the sensor system is configured to analyze the fluid sample, further comprising a mass exchanger in fluidic communication with the flow system and the sampling system, wherein the mass exchanger is configured to condition the fluid sample to produce a conditioned fluid sample for analysis by the sensor system, wherein the conditioned fluid sample is flowed through the mass exchanger.
2 more elements
A system comprising:
- a flow system including a pump system and a valve system, wherein the pump system and the valve system are in fluidic communication;
- a sampling system in fluidic communication with the flow system;
- a reactor comprising a fluid, wherein the sampling system is in fluidic communication with the reactor, wherein in a first configuration of the flow system the fluid sample is flowed from the reactor to the pump system through the valve system at a first flow rate;
- and
- a sensor system in fluidic communication with the flow system, wherein in a second configuration of the valve system the fluid sample is flowed from the pump system to the sensor system at a second flow rate, wherein the sensor system is configured to analyze the fluid sample, further comprising a mass exchanger in fluidic communication with the flow system and the sensor system, wherein the mass exchanger is configured to condition the fluid sample to produce a conditioned fluid sample for analysis by the sensor system or a mass exchanger in fluidic communication with the flow system and the separation system, wherein the mass exchanger is configured to condition the separated fluid sample to produce a conditioned fluid sample for analysis by the sensor system or a mass exchanger in fluidic communication with the separation system and the sensor system, wherein the mass exchanger is configured to condition the separated fluid sample to produce a conditioned fluid sample for analysis by the sensor system or all of these.
2 more elements
Dependencies are inferred from the claim text.