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US 11,959,135 B2Granted
Coupling method
AssigneeOxford Nanopore Technologies PLC
InventorsJames Anthony Clarke, James White, John Milton, Clive Gavin Brown
Life of this patent
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Abstract
The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
Claims11
A method for enhancing a target polynucleotide concentration on a surface during nanopore sequencing, the method comprising:
- (i) contacting a target polynucleotide construct to a surface comprising a nanopore, and a solid-state membrane supporting a lipid bilayer, wherein the target polynucleotide construct comprises:
- (a) a single stranded DNA (ssDNA) leader;
- (b) a target polynucleotide;
- and
- (c) one or more reactive groups that interact with the lipid bilayer, under conditions under which the one or more reactive groups interacts with the lipid bilayer and tethers the target polynucleotide construct to the lipid bilayer resulting in enhanced concentration of the target polynucleotide on the surface in proximity to the nanopore;
- and
- (ii) detecting the target polynucleotide using the nanopore.
4 more elements
Dependencies are inferred from the claim text.