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US 11,959,126 B2Granted
Microfluidic system for amplifying and detecting polynucleotides in parallel
AssigneeHandyLab, Inc.
InventorsKalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
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Abstract
The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
Claims18
A method, comprising:
- providing a multi-lane microfluidic cartridge, each lane comprising an amplification region within which amplification of polynucleotides is carried out;
- providing an instrument, the instrument comprising:
- a frame,
- a drawer configured to move relative to the frame, wherein the drawer comprises:
- an area configured to receive the multi-lane microfluidic cartridge,
- a registration member configured to facilitate placement of the multi-lane microfluidic cartridge into the drawer in a single orientation,
- a cutaway configured to facilitate removal of the multi-lane microfluidic cartridge from the drawer after analysis,
- a plurality of separately controllable heat sources configured to deliver localized heat to selected regions of the multi-lane microfluidic cartridge at specific times, and
- electrical connectors configured to transmit electrical signals to the plurality of separately controllable heat sources during analysis;
- one or more processors configured to control the plurality of separately controllable heat sources, a heat source of the plurality of separately controllable heat sources configured to direct heat to an amplification region of a lane of the multi-lane microfluidic cartridge when the multi-lane microfluidic cartridge is received in the instrument;
- and
- controlling the plurality of separately controllable heat sources to perform amplification of polynucleotides within the amplification region of the multi-lane microfluidic cartridge.
9 more elements
Dependencies are inferred from the claim text.