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US 11,959,119 B2Granted
Methods and systems for starch based propagation of a microorganism
AssigneePOET Research, Inc.
InventorsCory James Sarks, Malgorzata M. Slupska, David Charles Carlson, Benjamin M. Gerdes, Neelakantam V. Narendranath, David D. Bushong
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Abstract
Systems and methods that include starch derived from grain as a carbon source for propagation of microorganisms.
Claims20
A method of producing a biochemical, the method comprising:
- providing a ground grain slurry comprising starch;
- hydrolyzing the starch from the ground grain slurry to produce a hydrolysate comprising at least one or more monosaccharides;
- providing a propagation composition comprising at least a portion of the hydrolysate comprising the one or more monosaccharides, and a first cell mass of a microorganism;
- exposing the propagation composition to conditions to propagate the first cell mass of the microorganism into a second cell mass of the microorganism, wherein the second cell mass is greater than the first cell mass of the microorganism;
- providing a lignocellulosic hydrolysate comprising:
- one or more saccharides derived from lignocellulosic feedstock, and
- one or more toxins that are toxic to the microorganism;
- providing a fermentation composition comprising:
- at least a portion of the lignocellulosic hydrolysate, and
- at least a portion of the second cell mass of microorganism;
- and
- exposing the fermentation composition to conditions so that the at least a portion of the second cell mass of microorganism converts saccharides in the fermentation composition into a biochemical.
9 more elements
Dependencies are inferred from the claim text.