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US 11,959,117 B2Granted
Methods and compositions for the enzymatic production of pseudouridine triphosphate
AssigneeNature's Toolbox, Inc.
InventorsAlexander Koglin, Michael Humbert, Matthias Strieker, Grant Eliason
Life of this patent
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Abstract
The present invention includes novel systems, methods, and compositions for the enzymatic/chemical production of pseudouridine (Ψ) and its variants, such as N1-methyl-pseudouridine-5′-triphosphate (m1ΨTP).
Claims22
A system for the production of pseudouridine compounds comprising:
- an isolated uracil nucleobase, and isolated ribose-5-phosphate, wherein the uracil nucleobase with the ribose-5-phosphate are converted to form pseudouridine-5′-monophosphate (ΨMP), wherein the formation of ΨMP is catalyzed by pseudouridine-5′-phosphate glycosidase, or a functional fragment or variant thereof;
- a protecting agent that reacts with the hydroxyl groups of said ΨMP forming a protected ΨMP;
- a methylating agent that reacts with the protected ΨMP, forming a protected N1-methyl -pseudouridine-5′-monophosphate (protected m1ΨMP) compound;
- a deprotection agent that reacts with the protected m1ΨMP forming N1-methyl-pseudouridine-5′-monophosphate (m1ΨMP);
- and
- a phosphorylating agent that catalyzes the sequential phosphorylation of m1MP forming N1-methyl-pseudouridine-5′-diphosphate (m1ΨTP), and N1-methyl-pseudouridine-5′-triphosphate (m1ΨTP).
3 more elements
A system for the production of pseudouridine compounds comprising
- an isolated uracil nucleobase, and isolated ribose-5-phosphate, wherein said uracil nucleobase with a ribose-5-phosphate are converted to form pseudouridine-5′-monophosphate (ΨMP), wherein the enzymatic formation of ΨMP is catalyzed by pseudouridine-5′-phosphate glycosidase, or a functional fragment or variant thereof;
- and
- a protecting agent that reacts with the hydroxyl groups of said ΨMP forming a protected ΨMP.
A system for the production of pseudouridine compounds comprising
- an isolated uracil nucleobase, and isolated ribose-5-phosphate, wherein said uracil nucleobase with a ribose-5-phosphate are converted to form pseudouridine-5′-monophosphate (ΨMP), wherein the formation of ΨMP is catalyzed by pseudouridine-5′-phosphate glycosidase or a functional fragment or variant thereof;
- a protecting agent that reacts with the hydroxyl groups of said ΨMP forming a protected ΨMP;
- and
- a methylating agent that reacts with the protected ΨMP, forming a protected N1-methyl-pseudouridine-5′-monophosphate (protected m1ΨMP) compound.
1 more element
A system for the production of pseudouridine compounds comprising
- an isolated uracil nucleobase, and isolated ribose-5-phosphate, wherein said uracil nucleobase with a ribose-5-phosphate are converted to form pseudouridine-5′-monophosphate (ΨMP), wherein the formation of ΨMP is catalyzed by pseudouridine-5′-phosphate glycosidase or a functional fragment or variant thereof;
- a protecting agent that reacts with the hydroxyl groups of said ΨMP forming a protected ΨMP;
- a methylating agent that reacts with the protected ΨMP, forming a protected N1-methyl-pseudouridine-5′-monophosphate (protected m1MP) compound;
- and
- a deprotection agent that reacts with the protected m1ΨMP forming N1-methyl-pseudouridine-5′-monophosphate (m1MP).
2 more elements
Dependencies are inferred from the claim text.