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US 11,959,110 B2Granted
Recombinant prothrombin analogues and uses thereof
AssigneeCleveland State University
InventorsMichael Kalafatis, Joseph Wiencek
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Abstract
During the process of coagulation, prothrombin is activated to α-thrombin by prothrombinase. Key residues in the structure of prothrombin allow for modulation of the activation of prothrombin. In certain embodiments, a recombinant prothrombin with at least one point mutation or deletion is provided.
Claims6
A method of modulating coagulation in a subject who has or is at risk of having unwanted coagulation, the method comprising:
- identifying an individual susceptible to unwanted coagulation;
- and
- administering an impotent recombinant prothrombin molecule wherein the impotent recombinant prothrombin molecule comprises a deletion of amino acid residues Ser478, Leu480, and Gln481 or a substitution of Ser478, Leu480, and Gln481 with alanine, to the individual.
A method for producing an impotent prothrombin molecule having an amino acid sequence of prothrombin with an amino acid modification from residue 472 to 487, the method comprising:
- (a) introducing into a host cell an expression vector which contains a nucleotide sequence which encodes a prothrombin molecule wherein the prothrombin molecule comprises a deletion of amino acid residues Ser478, Leu480, and Gln481 or a substitution of Ser478, Leu480, and Gln481 with alanine;
- (b) culturing the host cell in an appropriate medium;
- and
- (c) isolating the polypeptide product encoded by the expression vector.
1 more element
Dependencies are inferred from the claim text.