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US 11,959,105 B2Granted
Polymerizing enzymes for sequencing reactions
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Manjula Pandey
Life of this patent
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Abstract
Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.
Claims17
A modified recombinant DNA polymerase based on SEQ ID NO:
- 2, comprising a first segment and a second segment, wherein:
- the first segment consists of an amino acid sequence corresponding to a first reference segment, said first reference segment consisting of amino acids 197-271 of SEQ ID NO:
- 2, wherein the amino acid sequence of the first segment is at least 80%, 80-90%, 90-95%, or at least 95% identical to the amino acid sequence of the first reference segment, and wherein the first segment has a higher isoelectric point than the first reference segment;
- and
- the second segment consists of an amino acid sequence corresponding to a second reference segment, said second reference segment consisting of amino acids 443-528 of SEQ ID NO:
- 2, wherein the amino acid sequence of the second segment is at least 80%, 80-90%, 90-95%, or at least 95% identical to the amino acid sequence of the second reference segment, and wherein the second segment has a higher isoelectric point than the second reference segment.
3 more elements
Dependencies are inferred from the claim text.