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US 11,959,104 B2Granted
Methods of differentiating stem cell-derived ectodermal lineage precursors
AssigneeMemorial Sloan Kettering Cancer Center
InventorsLorenz Studer, Bastian Zimmer, Jason Tchieu
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Abstract
The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into neural crest, cranial placode or non-neuro ectoderm precursors, and cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating neurodegenerative and pituitary disorders.
Claims17
An in vitro method for inducing differentiation of stem cells, comprising:
- contacting stem cells with at least one inhibitor of transforming growth factor beta (TGFβ)/Activin-Nodal signaling and at least one activator of bone morphogenetic protein (BMP) signaling for at least about 2 days, and
- contacting the cells with at least one inhibitor of fibroblast growth factor (FGF) signaling to obtain a population of differentiated cells expressing at least one non-neural ectoderm lineage marker.
Dependencies are inferred from the claim text.