Method for inducing pluripotent stem cells to differentiate into somatic cells
AssigneesOsaka University, Kyoto University
InventorsKiyotoshi Sekiguchi, Fumi Ebisu, Hidetoshi Sakurai, Mingming Zhao, Megumu Saito
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Abstract
Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.
Claims9
A method for inducing pluripotent stem cells to differentiate into somatic cells, the method comprising bringing pluripotent stem cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan in a culture medium containing a heparin binding growth factor and a canonical Wnt signal activator,
- wherein the somatic cells are mesoderm-derived somatic cells, and
- wherein the heparin binding growth factor is one or more selected from the group consisting of bone morphogenetic protein 4, activin A, basic fibroblast growth factor, hepatocyte growth factor and vascular endothelial growth factor.
Dependencies are inferred from the claim text.