Methods for selective inhibition of pluripotent stem cells
AssigneesThe Medical College of Wisconsin, Inc., The Government of the United States of America as Represented by the Secretary of the Department of Health and Human Services NIH, Office of Technology Transfer
InventorsRebekah L. Gundry, Kenneth R. Boheler, Erin M. Kropp
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Abstract
Provided herein are methods of reducing or eliminating undifferentiated pluripotent stem cells, where the methods comprise contacting an effective amount of a compound to a heterogeneous cell population or sample comprising or suspected of comprising differentiated cell types and undifferentiated pluripotent stem cells, whereby the contacting selectively reduces or eliminates undifferentiated pluripotent stem cells from the cell population or sample. Also provided are methods for obtaining a population of stem cell-derived cell types substantially free of undifferentiated pluripotent stem cells as well as isolated populations of such of stem cell-derived cell types.
Claims7
A method of obtaining a population of stem cell-derived cell types substantially free of undifferentiated pluripotent stem cells, the method comprising
- (a) inducing undifferentiated pluripotent stem cells to differentiate or partially differentiate into one or more stem cell-derived cell types;
- (b) contacting an effective amount of a compound to the induced cell population, wherein the compound is a nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, whereby the contacting selectively reduces or eliminates undifferentiated pluripotent stem cells from the induced cell population;
- and
- (c) isolating the contacted cells to obtain a population of one or more stem cell-derived cell types, wherein the population is substantially free of undifferentiated pluripotent stem cells.
Dependencies are inferred from the claim text.