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US 11,959,093 B2Granted
Integration sites and uses thereof
AssigneeApplied Stemcell, Inc.
InventorsLing-Jie Kong, Ruby Yanru Tsai, Xiuling Chi
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Abstract
The present disclosure provides compositions and methods for enhanced expression of exogenous genes in eukaryotic cells. The method involves introducing into a mammalian cell an exogenous nucleic acid. wherein the exogenous nucleic acid intearates into a locus of the genome that comprises an extended methylation-free CpG island. Also provided are chromosomal loci, sequences for enhanced and stable expression of exogenous genes.
Claims10
A method, comprising:
- introducing an exogenous nucleic acid into an isolated mammalian cell in vitro;
- and
- integrating the exogenous nucleic acid into a locus of the genome of the isolated mammalian cell using
- (i) a Cas nuclease and a gRNA targeting the locus of the genome, or
- (ii) a recombinase,
- wherein the locus comprising an extended methylation-free CpG island and a nucleotide sequence at least 90% identical to SEQ ID NO:1.
3 more elements
Dependencies are inferred from the claim text.