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US 11,959,089 B2Granted
Recombinant LAC polynucleotides and uses thereof to increase production of C-lignin in plants
AssigneeUniversity of North Texas
InventorsRichard A. Dixon, Fang Chen, Chunliu Zhuo
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Abstract
The disclosure provides recombinant LAC polynucleotides encoding a laccase (LAC) polypeptide capable of polymerizing caffeyl alcohol, vectors and cells including the recombinant LAC polynucleotide. The disclosure also provides transgenic plants including cells having the recombinant LAC polynucleotide of the present disclosure and methods of increasing production of C-lignin in a plant.
Claims19
A recombinant polynucleotide comprising:
- a LAC polynucleotide encoding a laccase (LAC) polypeptide capable of polymerizing caffeyl alcohol, the LAC polynucleotide encoding a polypeptide having a peptide sequence that is 100% identical to LAC8 from Cleome hassleriana (ChLAC8) having SEQ ID NO:
- 3, or is a variant thereof that is at least 99% identical to SEQ ID NO:3, and having a glutamine residue in an active site position of the polypeptide configured to be in substantial proximity to interact with a 3 hydroxyl group of a caffeyl alcohol bound in an active site of the polypeptide;
- and at least one heterologous polynucleotide sequence operatively linked to the LAC polynucleotide.
A vector comprising the recombinant polynucleotide of clam 1.
Dependencies are inferred from the claim text.