Dual transit peptides for targeting polypeptides

A recombinant chimeric nucleic acid molecule comprising a nucleic acid sequence encoding a dual transit peptide operably linked to a heterologous nucleic acid sequence encoding a heterologous polypeptide of interest,

• wherein said dual transit peptide is from the genus Amaranthus and translocates the polypeptide of interest to a chloroplast or a mitochondrion,
• wherein said dual transit peptide comprises an amino acid sequence having 100% sequence identity to SEQ ID NO:
• 1,
• wherein said heterologous polypeptide of interest is a polypeptide which, when overexpressed in a plant, confers herbicide tolerance to said plant,
• wherein said heterologous polypeptide of interest is from a prokaryote,
• wherein said polypeptide has protoporphyrinogen oxidase (PPO) activity and confers tolerance to PPO-inhibiting herbicides, and
• wherein said polypeptide having PPO activity comprises an amino acid sequence having 100% sequence identity to SEQ ID NO:
• 320.