Regulatory elements from lamium leaf distortion associated virus (LLDAV)
AssigneesPioneer Hi-Bred International, Inc., Corteva Agriscience LLC
InventorsScott Diehn, Michelle Van Allen, Albert L Lu
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Abstract
The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for regulatory elements from Lamium Leaf Distortion Associated Virus. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory element sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the regulatory elements of the present disclosure.
Claims11
A recombinant polynucleotide comprising one or more enhancer sequences operably linked to a regulatory element having promoter activity operably linked to a heterologous polynucleotide, wherein the regulatory element comprises a polynucleotide having the nucleic acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, or SEQ ID NO:
- 5, wherein the recombinant polynucleotide further comprises one or more intron sequences operably linked to the regulatory element, and wherein the enhancer sequence is derived from a virus and the intron sequence is derived from Zea mays.
A DNA construct comprising
- (a) one or more enhancer sequences operably linked to a regulatory element polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and SEQ ID NO:
- 5 operably linked to one or more intron sequences, and
- (b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide, wherein the regulatory element polynucleotide has promoter activity.
A method for expressing a heterologous nucleotide sequence in a maize plant or plant cell comprising introducing into a maize plant or plant cell a DNA construct comprising:
- (a) one or more enhancer sequences operably linked to a regulatory element polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and SEQ ID NO:
- 5 operably linked to one or more intron sequences, and
- (b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide, wherein the regulatory element polynucleotide has promoter activity;
- and
- (c) wherein the heterologous transcribable nucleotide sequence is expressed in said maize plant or plant cell.
Dependencies are inferred from the claim text.