Nucleic acid construct
AssigneeAutolus Limited
InventorsShaun Cordoba, James Sillibourne, Martin Pulé
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Abstract
The present invention provides a nucleic acid construct comprising: a first nucleotide sequence of interest (NOI1); a frame-slip motif or a translational readthrough motif (FSM/TRM); and a second nucleotide sequence of interest (NOI2). The invention also provides vectors and cells expressing such a construct. The invention also provides a method for modulating the relative expression of two transgenes in a nucleic acid construct which comprises the step of including a frame-slip motif or a translational readthrough motif between the two transgenes in order to reduce the expression of the downstream transgene.
Claims21
A nucleic acid construct comprising:
- a first nucleotide sequence of interest (NOI1), a translational readthrough motif (TRM), and a second nucleotide sequence of interest (NOI2), and
- a nucleotide sequence encoding a cleavage site (CL), so that NOI1 and NOI2 are expressed as separate proteins,
- wherein the (TRM) comprises the sequence STOP-CUAG or STOP-CAAUUA, in which “STOP” is a stop codon.
A nucleic acid construct comprising:
- a first nucleotide sequence of interest (NOI1), a translational readthrough motif (TRM), and a second nucleotide sequence of interest (NOI2), and
- a nucleotide sequence encoding a cleavage site (CL), so that NOI1 and NOI2 are expressed as separate proteins,
- wherein NOI2 encodes a cytokine, chemokine or toxin.
A nucleic acid construct comprising:
- a first nucleotide sequence of interest (NOI1), a translational readthrough motif (TRM) and a second nucleotide sequence of interest (NOI2),
- wherein the nucleic acid construct is capable of producing two products when expressed in a cell:
- a) a first product encoded by NOI1 alone, and
- b) a second product, encoded by NOI1 and NOI2, which is produced when translational readthrough occurs, and
- wherein the first and/or second product is a chimeric antigen receptor (CAR).
Dependencies are inferred from the claim text.