Process for isolating nucleic acids from sample materials
AssigneeAxagarius GmbH & Co. KG
InventorsAndreas von Bohl, Yvonne Schuell
Life of this patent
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Abstract
The invention relates to a process for isolating nucleic acids, such as DNA and RNA, using a non-alcoholic and non-chaotropic binding solution, and to the binding solutions and kits suitable for such a process.
Claims22
A process for isolating nucleic acids from a biological sample, comprising:
- (a) lysing the biological sample,
- (b) adding a binding solution being free of chaotropic salts and comprising at least one organic solvent selected from the group consisting of cyclic C2-4 alkylene carbonates, C2-3 alkylene glycol diacetates, and derivatives thereof, at least one non-ionic surfactant, and nucleic acid-binding carboxylated magnetic particles as a nucleic acid-binding solid phase to the lysed sample, and separating the solid phase with the nucleic acids directly bound thereto,
- (c) washing the separated solid phase including the nucleic acids bound thereto once or several times with identical or different washing solutions, and
- (d) desorbing the nucleic acids from the solid phase by adding an aqueous elution buffer.
A binding solution for isolating nucleic acids from a biological sample, said binding solution being free of chaotropic salts and said binding solution comprising at least one organic solvent selected from the group consisting of cyclic C2-4 alkylene carbonates, C2-3 alkylene glycol diacetates, and derivatives thereof, at least one non-ionic surfactant, and nucleic acid-direct-binding carboxylated magnetic particles as a nucleic acid-binding solid phase.
A kit for isolating nucleic acids from a biological sample, comprising a binding solution that is free of chaotropic salts and said binding solution comprising at least one organic solvent selected from the group consisting of cyclic C2-4 alkylene carbonates, C2-3 alkylene glycol diacetates, and derivatives thereof, at least one non-ionic surfactant, and nucleic acid-direct-binding carboxylated magnetic particles as a nucleic acid-binding solid phase.
Dependencies are inferred from the claim text.