Self-immolative plasmid backbone
AssigneeProteoNic Biotechnology IP B.V.
InventorsBart Marinus Engels, Raymond Michael Dimphena Verhaert, Maurice Wilhelmus Van der Heijden
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Abstract
The invention relates to a method for separating a polynucleotide insert from a polynucleotide vector backbone. The backbone has a plurality of cleavage sites distributed such that the backbone is converted into fragments when the sites are cleaved. This allows straightforward separation of the insert from the backbone. The invention also relates to backbones for use in such a method, and to plasmids and kits comprising such backbones.
Claims19
A method for separating a polynucleotide insert from a polynucleotide vector backbone, the method comprising the steps of
- i) providing a recombinant polynucleotide vector comprising the insert and the vector backbone, wherein the vector backbone comprises a first plurality of cleavage sites that divide the vector backbone into fragments, wherein all fragments are selected from a length of at most 1000 bp and at least 40 bp;
- ii) contacting the recombinant vector with cleavage means capable of specifically cleaving the first plurality of cleavage sites to produce backbone fragments and the insert;
- and optionally,
- iii) separating the insert from the backbone fragments of step ii).
Dependencies are inferred from the claim text.