Methods and devices for electroporation

A process for electroporation of biological cells, comprising:

• providing a sample container for electroporation, the sample container having an insulator chamber for containing a cell sample, a first electrode and a second electrode providing electrical connection for electroporation, and a first conductive medium layer for forming a surface which is separated from the first electrode and the second electrode;
• arranging biological cells to form at least one cell monolayer on the surface of the first conductive medium layer, the at least one cell monolayer being separated from the first electrode and the second electrode, wherein the first electrode is in a cover configured to removably cover the insulator chamber, and the insulator chamber is configured to contain a groove on a top of one side of the insulator chamber, the cover being shaped to fit with the groove to seal the insulator chamber, the first electrode covering an inner rim of the groove on the side of the insulator chamber, and an outer rim of the groove being higher than the inner rim of the groove;
• treating the biological cells in the at least one cell monolayer with a predetermined electrical pulse, the predetermined electrical pulse being generated by a pulse generator;
• pushing extra sample out by sealing of the sample container;
• and
• containing the extra sample pushed out by the sealing of the sample container in the groove.

A method for electroporation of biological cells, comprising:

• providing a sample container for electroporation, the sample container having an insulator chamber for forming the body of the container to hold cells, a first electrode and a second electrode providing electrical connection for electroporation, wherein the insulator chamber includes an excessive-receiving groove on a top of one side of the insulator chamber, and the first electrode is in a cover configured to removably cover an inner rim of the excessive-receiving groove on a side of the insulator chamber to seal the insulator chamber;
• filling the sample container with a sample containing biological cells in a volume slightly larger than the sealed volume in the sample container to form a convex meniscus surface in the insulator chamber;
• closing down the first electrode on the inner rim to push out excess liquid and seal the chamber, wherein the excess liquid flows to the excess-receiving groove;
• and
• delivering an electrical pulse to perform electroporation, wherein the excess liquid in the excess-receiving groove is discardable after electroporation.