CAR-T cells targeting glioma stem cells for the treatment of glioblastoma multiforme
AssigneeH. Lee Moffitt Cancer Center and Research Institute, Inc.
InventorsJames Liu, Daniel Abate-Daga
Life of this patent
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Abstract
Disclosed are compositions and methods for targeted treatment of glioblastoma multiforme (GBM). In particular, chimeric antigen receptor (CAR) polypeptides are disclosed that can be used with adoptive cell transfer to target and kill Glioblastoma Stem Cells (GSCs). Also disclosed are immune effector cells, such as T cells or Natural Killer (NK) cells, that are engineered to express these CARs. Therefore, also disclosed are methods of providing an anti-tumor immunity in a subject with Glioblastoma Stem Cells (GSCs) that involves adoptive transfer of the disclosed immune effector cells engineered to express the disclosed CARs.
Claims14
A polypeptide, comprising a Glioblastoma Stem Cells (GSC) antigen binding domain, a transmembrane domain, a co-stimulatory signaling region, and optionally an intracellular signaling domain, wherein the GSC antigen binding domain is a polypeptide selected from the group consisting of
- (SEQ ID NO:
- 15)
- SSQPFWS-GGGGSGGGGSGGGGS-AWEFYFP-GSTSGSGKPGSGEGSTKG
- (SEQ ID NO:
- 17)
- AWEFYFP-GGGGSGGGGSGGGGS-SSQPFWS-GSTSGSGKPGSGEGSTKG
- (SEQ ID NO:
- 19)
- AWTTTFP-GGGGSGGGGSGGGGS-SSQPFWS-GSTSGSGKPGSGEGSTKG.
- (SEQ ID NO:
- 21)
- AWTWTLP-GGGGSGGGGSGGGGS-SSQPFWS-GSTSGSGKPGSGEGSTKG
- (SEQ ID NO:
- 23)
- EWLFSMP-GGGGSGGGGSGGGGS-SSQPFWS-GSTSGSGKPGSGEGSTKG
- (SEQ ID NO:
- 25)
- GWFYTFP-GGGGSGGGGSGGGGS-SSQPFWS-GSTSGSGKPGSGEGSTKG
- (SEQ ID NO:
- 27)
- NWYWVYP-GGGGSGGGGSGGGGS-SSQPFWS-GSTSGSGKPGSGEGSTKG.
Dependencies are inferred from the claim text.