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US 11,958,880 B2Granted
Means and methods for site-specific functionalization of polypeptides
AssigneesLudwig-Maximilians-Universitat Munchen, Forschungsverbund Berlin E.V.
InventorsHeinrich Leonhardt, Jonas Helma, Dominik Schumacher, Christian Hackenberger
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Abstract
The present invention provides means and methods for equipping a polypeptide of interest at its C-terminus with a versatile adaptor amino acid that allows the functionalization of the polypeptide of interest.
Claims16
A method for the production of a polypeptide comprising
- (a) introducing or adding at the C-terminus of said polypeptide a recognition sequence for tubulin tyrosine ligase, wherein the recognition sequence comprises the amino acid sequence X4X3X2X1 (SEQ ID No. 11), wherein X4 is E, D, A, K, or P X3 is G, S, A, V or F, X2 is E, D or C and X1 is E;
- and
- (b) contacting the polypeptide obtained in step (a) in the presence of tubulin tyrosine ligase and a tyrosine derivative under conditions suitable for the tubulin tyrosine ligase to tyrosinate said polypeptide with said tyrosine derivative,
- wherein said polypeptide is an antibody or fragment thereof,
- wherein said tyrosine derivative is tyrosine derivative 1:
2 more elements
A method for the production of a polypeptide comprising
- (a′) introducing or adding at the C-terminus of a polypeptide a recognition sequence for tubulin tyrosine ligase, wherein the recognition sequence comprises the amino acid sequence X4X3X2X1 (SEQ ID No. 11), wherein X4 is E, D, A, K, or P X3 is G, S, A, V or F, X2 is E, D or C and X1 is E;
- and
- (b′) contacting the polypeptide obtained in step (a′) in the presence of tubulin tyrosine ligase and a tyrosine derivative conjugated to a moiety under conditions suitable for the tubulin tyrosine ligase to tyrosinate said polypeptide with said tyrosine derivative conjugated to said moiety,
- wherein said polypeptide is an antibody or fragment thereof,
- wherein said tyrosine derivative is tyrosine derivative 1:
2 more elements
Dependencies are inferred from the claim text.