Binding competitors for use in macrophilin-binding pharmaceutical assays and methods of use thereof
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsTie Wei, Zhu Teng, Martin Drinan, Jie Li
Life of this patent
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Abstract
The present disclosure includes compounds, kits, and assay procedures for use in determining the levels of certain types of drugs in samples that contain specific binding proteins for the drugs. The present disclosure includes analog compounds useful for displacing the drugs from their endogenous binding proteins, and kits including same, as well as methods that utilize these displacers as binding competitors in pharmaceutical assays.
Claims20
A composition comprising at least one of:
- (a) the compound represented by Formula IV:
- and
- (b) the compound represented by Formula VI:
A method of determining the presence of a macrophilin-binding pharmaceutical in a sample, the method comprising the steps of:
- adding to the sample a binding competitor to displace the macrophilin-binding pharmaceutical from its immunophilin complexes, wherein the binding competitor is at least one of:
- (a) the compound represented by Formula IV:
- (b) the compound represented by Formula V:
- (c) the compound represented by Formula VI:
- or
- (d) the compound represented by Formula VII:
- adding a macrophilin-binding pharmaceutical-specific binding partner that binds to the pharmaceutical to form a binding partner/pharmaceutical complex, and wherein the binding partner does not significantly bind to the binding competitor;
- and
- detecting the binding partner/pharmaceutical complex and correlating the detection with an amount of pharmaceutical present in the sample.
A kit for the detection of the presence of a macrophilin-binding pharmaceutical in a sample, the kit comprising:
- a binding competitor that displaces the macrophilin-binding pharmaceutical from its immunophilin complexes, wherein the binding competitor is at least one of:
- (a) the compound represented by Formula IV:
- (b) the compound represented by Formula V:
- (c) the compound represented by Formula VI:
- or
- (d) the compound represented by Formula VII:
- and
- a macrophilin-binding pharmaceutical-specific binding partner that binds to the pharmaceutical for detection of a binding partner/pharmaceutical complex, and wherein the binding partner does not significantly bind to the binding competitor.
Dependencies are inferred from the claim text.