Phenotype profile of human retinal progenitor cells
AssigneeThe Schepens Eye Research Institute, Inc.
InventorsMichael J. Young, Petr Y. Baranov
Life of this patent
Estimating the term…
Abstract
The present invention relates to substantially homogenous populations of human retinal progenitor cells having the following positive surface markers: SSEA4, CD73, PTK7 and PSA-NCAM. The invention also relates to method for preparing such substantially homogeneous cell populations from human tissue using cell sorting techniques.
Claims12
An expanded population of human retinal progenitor cells having been expanded in from about 10 ng/ml to about 20 ng/ml epidermal growth factor and from about 10 ng/ml to about 20 ng/ml basic fibroblast growth factor in low oxygen conditions consisting of about 3% oxygen for at least 6 hours and wherein at least 80% of the population of the cells express the following identifiable markers SOX2, Recoverin, Otx2, CD73, PCNA, Beta3tubulin, NF200, Nestin, and PTK7 but not expressing CD15 and CD133, and wherein the population is expanded for at least 8 passages.
An expanded population of human retinal progenitor cells having been expanded in from about 10 ng/ml to about 20 ng/ml epidermal growth factor and from about 10 ng/ml to about 20 ng/ml basic fibroblast growth factor in low oxygen conditions consisting of about 3% oxygen for at least 6 hours and wherein at least 90% of the population of the cells express the following identifiable markers SOX2, Recoverin, CD73, Beta3tubulin, NF200, Nestin, and PTK7 but not expressing CD15 and CD133, and wherein the population is expanded for at least 8 passages.
Dependencies are inferred from the claim text.