Methods and compositions of stable phenylephrine formulations

A method of dilating a pupil of a subject in need thereof, the method comprising storing a comprising R-phenylephrine hydrochloride and an aqueous buffer at a temperature between −10 to 10 degree Celsius prior to use and subsequently administering the composition to an eye of the subject, wherein the composition has a pH of fromabout 6.0 to about 6.7 when stored prior to administration, and wherein the composition comprises R-phenylephrine hydrochloride having an initial chiral purity of at least 95% enantiomeric excess (ee) as determined by chiral column chromatography at a time prior to storage and a chiral purity of at least 95% (ee) as determined by chiral chromatography at the time of use after storage when administered to the pupil of the subject, and wherein storing the composition at a temperature between −10 to 10 degrees Celsium maintains the chiral purity of at least 95% (ee) as determined by chiral column chromatography for a period of at least 6 months storage.