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AssigneeBASF Plant Science GmbH
InventorsXinmin Deng, Kristin Gray, Jakir Hasan, Keith Horton
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Various embodiments disclosed relate to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, such as canola, transgenically modified to produce seed oil comprising at least one of EPA, DHA and DPA. Transgenic Brassica plants, such as transgenic canola, are subjected to an environment which has an average daily day-night temperature difference of at least 13° C. during the transgenic plant's period of seed maturation. The seed oil is at least 5 wt % EPA. The seed oil is at least 1 wt % DPA. The seed oil is at least 0.2 wt % DHA. The seed oil is at least 5.2 wt % a mixture of EPA and DHA. The seed oil is at least 6 wt % long chain omega-3 fatty acids.
A method of increasing the proportion of long-chain omega-3 fatty acids in seed oil produced by a plurality of transgenic Brassica napus oilseed plants, comprising growing said transgenic Brassica napus oilseed plants at a daytime temperature of about 25° C., and a nighttime temperature of about 12° C., wherein said growing is for a period of seed maturation for said transgenic Brassica napus oilseed plants, wherein said period of seed maturation comprises BBCH-scale growth stage 6, BBCH-scale growth stage 7 and BBCH-scale growth stage 8 of Brassica napus oilseed plant growth stages, wherein said transgenic Brassica napus oilseed plants have been transgenically modified to produce said seed oil comprising at least one of EPA, DHA, and DPA, and wherein the proportion of long-chain omega-3 fatty acids in said seed oil produced by said transgenic Brassica napus oilseed plants is increased corresponding to controls of Brassica napus oilseed plants.
Dependencies are inferred from the claim text.