Quantitation of insulin-like growth factor-I and insulin-like growth factor-II with high-resolution mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsCory Bystrom, Shijun Sheng, Nigel Clarke, Richard Reitz
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Abstract
Methods are provided for determining the amount of an IGF-I and/or IGF-II protein in a sample using high resolution/high accuracy mass spectrometry. The methods generally comprise enriching an IGF-I and/or IGF-II protein in a sample, ionizing an IGF-I and/or IGF-II protein from the sample to generate IGF-I and/or IGF-II protein ions, and determining the amount of IGF-I and/or IGF-II protein ions with high resolution/high accuracy mass spectrometry.
Claims11
A method for determining an amount of insulin-like growth factor-II (IGF-II) protein in a sample, the method comprising:
- a. ionizing IGF-II in the sample to produce one or more ions detectable by mass sspectrometry;
- b. determining the amount of one or more of the ions comprising an ion with a mass-to-charge ratio selected from a group consisting of 934.69±2, 1068.07±2, 1245.92±2, and 1494.89±2 by mass spectrometry;
- and
- c. determining the amount of the IGF-II in the sample using the amount of the one or more ions determined in step b.
Dependencies are inferred from the claim text.