Methods and compositions for detecting misfolded proteins
AssigneeYale University
InventorsIrina Buhimschi, Catalin S. Buhimschi, Michael Choma, Hemant Tagare, Stephan Michael Jonas
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Abstract
Various aspects and embodiments of the present disclosure are directed to methods and compositions (e.g., kits) for the identification of subjects with misfolded proteins in their urine. For example, methods and compositions for determining that a urine sample from a pregnant woman contains or does not contain misfolded are provided. In some embodiments, the presence of misfolded proteins in a urine sample from a pregnant woman is an indication of preeclampsia.
Claims20
A method of detecting misfolded proteins in a biological sample obtained from a pregnant woman comprising:
- (a) applying a biological sample of a pregnant woman to a surface onto which a dye is adsorbed to produce a surface comprising a biological sample and the dye;
- (b) subjecting the surface to isoelectric focusing;
- and (c) determining the extent or rate of migration of the dye, wherein the extent or rate of migration of the dye is indicative of presence or absence of the misfolded proteins in the biological sample, such that the presence or absence of the misfolded proteins is detected.
Dependencies are inferred from the claim text.