Method for analyzing a biological sample containing biological cells, and analysis apparatus for implementing the analysis method

An analysis apparatus comprising:

• a flow cytometer comprising a measurement cell intended for the passage of biological cells of a biological sample to be analyzed and measuring means configured to measure cytometry parameters of the biological cells of the biological sample to be analyzed, and
• a processing unit configured to:
• determine, for each biological cell of the biological sample to be analyzed, a point in a N-dimensional space whose coordinates are defined depending on the cytometry parameters measured for the corresponding biological cell, where N is an integer greater than or equal to 3,
• cluster the points into different cell clusters depending on the cytometry parameters measured for each biological cell of the biological sample to be analyzed, so as to define a sample cluster file,
• perform an analysis action on the sample cluster file so as to detect at least one possible anomaly in the sample cluster file, wherein the analysis action comprises analyzing the cell clusters of the sample cluster file and detecting an anomaly if the presence or absence of at least one predetermined cell cluster is detected,
• compare the sample cluster file with reference cluster files, each of the reference cluster files being defined from cytometry parameters of a respective pathological or abnormal biological sample.

An analysis method for analyzing a biological sample containing biological cells including blood cells, the analysis method comprising the following steps:

• passage of the biological cells of the biological sample to be analyzed into a measuring cell of a flow cytometer,
• measurement of N cytometry parameters for each biological cell of the biological sample to be analyzed,
• determination, for each biological cell of the biological sample to be analyzed, of a point in a N-dimensional space whose coordinates are defined depending on the cytometry parameters measured for the corresponding biological cell, where N is an integer greater than or equal to 3,
• automatic clustering of the determined points into different cell clusters depending on the measured cytometry parameters, so as to define a sample cluster file,
• identification of cell populations defined by the different cell clusters of the sample cluster file,
• counting of the points of each cell cluster of the sample cluster file,
• analysis of the sample cluster file so as to detect at least one possible anomaly in the sample cluster file,
• comparison of the sample cluster file with reference cluster files, each of the reference cluster files being defined from cytometry parameters of a respective pathological or abnormal biological sample,
• wherein the comparison step is carried out only when the at least one possible anomaly is detected during the analysis step.

An analysis method for analyzing a biological sample containing biological cells including blood cells, the analysis method comprising the following steps:

• passage of the biological cells of the biological sample to be analyzed into a measuring cell of a flow cytometer,
• measurement of N cytometry parameters for each biological cell of the biological sample to be analyzed,
• determination, for each biological cell of the biological sample to be analyzed, of a point in a N-dimensional space whose coordinates are defined depending on the cytometry parameters measured for the corresponding biological cell, where N is an integer greater than or equal to 3,
• automatic clustering of the determined points into different cell clusters depending on the measured cytometry parameters, so as to define a sample cluster file,
• identification of cell populations defined by the different cell clusters of the sample cluster file,
• counting of the points of each cell cluster of the sample cluster file,
• comparison of the sample cluster file with reference cluster files, each of the reference cluster files being defined from cytometry parameters of a respective pathological or abnormal biological sample, and
• emitting an alarm message when the sample cluster file is at least partially identical or similar to a reference cluster file.