Light detection systems and methods of use thereof
AssigneeBecton, Dickinson and Company
InventorsJizuo Zou, Matthew Bahr, Eric D. Diebold
Life of this patent
Estimating the term…
Abstract
Light detection systems for measuring light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a light scatter detector, a brightfield photodetector and an optical adjustment component configured to convey light to the light scatter detector and to the brightfield photodetector. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having a light scatter detector, a brightfield photodetector and a beam splitter component are also provided.
Claims17
A particle analysis system configured to generate an image of particles in a sample, comprising:
- a flow cell configured to propagate the sample in a flow stream;
- an aspheric lens configured to receive light from the flow cell;
- a mirror configured to reflect light from the aspheric lens;
- a light scatter detector configured to obtain signals corresponding to structures within each particle in the sample;
- a brightfield photodetector configured to obtain signals corresponding to the shape and size of each particle in the sample, wherein the light scatter detector is positioned orthogonally with respect to the brightfield photodetector;
- a beam splitter configured to receive light from the mirror and transmit light to the light scatter detector and reflect light to the brightfield photodetector, wherein the beam splitter is configured to convey 10% or less of the light to the brightfield photodetector and 90% or more of the light to the light scatter detector;
- and
- a processor configured to generate the image of the particles in the sample based on the signals obtained by the light scatter detector and the brightfield photodetector.
A flow cytometer comprising:
- a light source;
- and
- a flow cell configured to propagate a sample in a flow stream;
- a light detection system configured to generate an image of particles in the sample, comprising:
- an aspheric lens configured to receive light from the flow cell;
- a mirror configured to reflect light from the aspheric lens;
- a light scatter detector configured to obtain signals corresponding to structures within each particle in the sample;
- a brightfield photodetector configured to obtain signals corresponding to the shape and size of each particle in the sample, wherein the light scatter detector is positioned orthogonally with respect to the brightfield photodetector;
- a beam splitter configured to receive light from the mirror and transmit light to the light scatter detector and reflect light to the brightfield photodetector, wherein the beam splitter is configured to convey 10% or less of the light to the brightfield photodetector and 90% or more of the light to the light scatter detector;
- and
- a processor configured to generate the image of the particles in the sample based on the signals obtained by the light scatter detector and the brightfield photodetector.
A method comprising generating an image of particles in a sample with a particle analysis system comprising:
- a flow cell configured to propagate the sample in a flow stream;
- an aspheric lens configured to receive light from the flow cell;
- a mirror configured to reflect light from the aspheric lens;
- a light scatter detector configured to obtain signals corresponding to structures within each particle in the sample;
- a brightfield photodetector configured to obtain signals corresponding to the shape and size of each particle in the sample, wherein the light scatter detector is positioned orthogonally with respect to the brightfield photodetector;
- a beam splitter configured to receive light from the mirror and transmit light to the light scatter detector and reflect light to the brightfield photodetector, wherein the beam splitter is configured to convey 10% or less of the light to the brightfield photodetector and 90% or more of the light to the light scatter detector;
- and
- a processor configured to generate the image of the particles in the sample based on the signals obtained by the light scatter detector and the brightfield photodetector.
Dependencies are inferred from the claim text.