Kits and methods for detecting cancer-related mutations
AssigneeNucleix Ltd.
InventorsDanny Frumkin, Adam Wasserstrom, Revital Knirsh
Life of this patent
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Abstract
Methods and kits for detection of cancer-related mutations in a DNA sample using enzymatic restriction and real-time PCR. A DNA sample is subjected to digestion with a restriction endonuclease to obtain restriction endonuclease-treated DNA, followed by co-amplification of a restriction locus comprising a cancer mutation site and a control locus. A ratio of signal intensities of the amplification products of the restriction locus and the control locus is used to detect the cancer-related mutation.
Claims20
A method for detecting a cancer-related mutation in a DNA sample, the method comprising:
- (a) subjecting the DNA sample to digestion with a restriction endonuclease to obtain restriction endonuclease-treated DNA;
- (b) co-amplifying from the restriction endonuclease-treated DNA a restriction locus comprising a cancer mutation site and a control locus, thereby generating an amplification product for each locus,
- (c) calculating a ratio between signal intensities of the amplification products of the restriction locus and the control locus;
- and
- (d) detecting the cancer-related mutation in the DNA sample by comparing the ratio calculated in step (c) to a predefined threshold ratio.
A kit for detecting cancer-related mutations in a DNA sample, the kit comprising:
- at least one restriction endonuclease for digesting a DNA sample;
- a plurality of primer pairs for co-amplification of at least one restriction locus comprising a cancer mutation site and at least one control locus following digestion with the restriction endonuclease;
- and
- a computer readable medium storing a computer software that directs a computer processor to detect a cancer-related mutation in the DNA sample based on a comparison of a ratio of signal intensities of the restriction locus and the control locus following amplification to a predefined threshold ratio.
A system for detecting cancer-related mutations in a DNA sample, the system comprising:
- at least one restriction endonuclease for digesting a DNA sample;
- a plurality of primer pairs for co-amplification of at least one restriction locus comprising a cancer mutation site and at least one control locus following digestion with the restriction endonuclease;
- and
- computer software stored on a computer readable medium that directs a computer processor to detect a cancer-related mutation in the DNA sample based on a comparison of a ratio of signal intensities of the restriction locus and the control locus following amplification to a predefined threshold ratio.
Dependencies are inferred from the claim text.