Dried compositions containing flap endonuclease
AssigneeGen-Probe Incorporated
InventorsPatrick Peterson, Tony Luu, Matthias Jost
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Abstract
There is disclosed a composition of an aqueous solution comprising, consisting or consisting essentially of a flap endonuclease, a bulking agent and an organic buffer, wherein the aqueous solution has an inorganic salt concentration of 5 mM or less and wherein the composition is substantially free of glycerol.
Claims21
A kit for use in performing a nucleic acid based assay, the kit comprising a first vessel containing adried composition, and a second vessel containing a reconstitution solution;
- wherein the dried composition comprises a bulking agent, a flap-endonuclease, dNTPs, a chelating agent, 3-(N-morpholino)propanesulfonic acid (MOPS) or tris(hydroxymethyl)aminomethane (Tris), and contains substantially no magnesium ions;
- and wherein the reconstitution solution comprises MgCl2 at a concentration from about 3.8 mM to about 4.4 mM.
A kit for use in performing a non-nucleic acid based assay, the kit comprising a first vessel containing a dried composition and a second vessel containing a reconstitution solution;
- wherein the dried composition comprises a bulking agent, a flap-endonuclease, a DNA polymerase, dNTPs, a chelating agent, 3-(N-morpholino)propanesulfonic acid (MOPS) or tris(hydroxymethyl)aminomethane (Tris), and contains substantially no magnesium ions;
- and wherein the reconstitution solution comprises MgCl2 at a concentration from about 3.8 mM to about 4.4 mM.
Dependencies are inferred from the claim text.