Methods and systems for analyzing nucleic acid molecules
AssigneeGuardant Health, Inc.
InventorsAndrew Kennedy, Stefanie Ann Ward Mortimer, Helmy Eltoukhy, AmirAli Talasaz, Diana Abdueva
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Abstract
The disclosure provides methods for processing nucleic acid populations containing different forms (e.g., RNA and DNA, single-stranded or double-stranded) and/or extents of modification (e.g., cytosine methylation, association with proteins). These methods accommodate multiple forms and/or modifications of nucleic acid in a sample, such that sequence information can be obtained for multiple forms. The methods also preserve the identity of multiple forms or modified states through processing and analysis, such that analysis of sequence can be combined with epigenetic analysis.
Claims21
A method of analyzing nucleic acids obtained from a subject having cancer or suspected of having cancer, comprising:
- (a) purifying cell-free nucleic acids from a bodily fluid sample obtained from the subject;
- (b) physically fractionating the cell-free nucleic acids to generate two or more partitions, wherein the physical fractionating comprises fractionating nucleic acids based on one or more characteristics, wherein the one or more characteristics comprises methylation status;
- and
- (c) sequencing at least a portion of nucleic acids from each of the two or more partitions to generate a set of sequencing reads.
Dependencies are inferred from the claim text.