Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
AssigneeSafeguard Biosystems Holdings Ltd.
InventorsHolger Klapproth, Nicolaas Smit
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Abstract
The present disclosure provides improved methods for bead beating and a bead beating system useful therefor. The bead beating system comprises a sample tube, beads, and a dry blocking agent, and methods for using the bead beating system to extract nucleic acids from cells containing the nucleic acids.
Claims28
A method for producing a lysate from cells contained in a blood sample, comprising agitating the blood sample within a bead beating system in the absence of a buffer, in the absence of a detergent, and in the presence of one or more endogenous blocking agents comprising urea under conditions sufficient to (i) lyse both a fungal pathogen and a bacterial pathogen and (ii) provide a lysate suitable for PCR amplification of DNA from the fungal pathogen, when present, and the bacterial pathogen, when present, said bead beating system comprising a bead beating tube and beads.
Dependencies are inferred from the claim text.