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US 11,952,612 B2Granted
Protease sensor molecules
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsHelen Dacres, Stephen Charles Trowell
Life of this patent
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Abstract
The present invention relates to sensors and methods for detecting bacterial proteases in a sample. In particular, the present invention relates to sensors and methods for detecting Pseudomonas spp. protease activity in a sample. The sensors and methods may be used to detect or predict spoilage of a dairy product. The invention also relates to methods for preparing samples for protease assays.
Claims16
A Pseudomonas spp. protease sensor molecule comprising:
- a target sequence comprising the amino acid sequence selected from SFMKKNQNTEI (SEQ ID NO:
- 45) and LQGSFMKKNQNTEIGSFE (SEQ ID NO:
- 72) and combinations thereof;
- and
- a chemiluminescent donor domain and an acceptor domain covalently attached to the target sequence,
- wherein cleavage of the target sequence by a Pseudomonas spp. protease produces a detectable change in resonance energy transfer.
3 more elements
Dependencies are inferred from the claim text.