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US 11,952,604 B2Granted
Enzymatic method for preparing Rebaudioside J
AssigneePepsiCo, Inc.
InventorsAlex Tao, Guoqing Li, Wenxia Wang, Leilei Zheng, Chunlei Zhu, Xiaoliang Liang, Kuikiu Chan
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Abstract
Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.
Claims12
An enzymatic method for preparing Rebaudioside J, the method comprising reacting Rebaudioside A with a rhamnosyl donor in a reaction system comprising:
- recombinant cells comprising a UDP-glycosyltransferase, a UDP-glycosyltransferase prepared therefrom, or both the recombinant cells comprising the UDP-glycosyltransferase and the UDP-glycosyltransferase prepared therefrom, wherein the UDP-glycosyltransferase comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO:
- 2, wherein Rebaudioside A is converted to Rebaudioside J at a conversion rate greater than 90%.
Dependencies are inferred from the claim text.