Variants of porcine trypsin
AssigneeSanofi-Aventis Deutschland GmbH
InventorsThomas Stillger, Sebastian Rissom, Claudia Feller, Andreas Vogel
Life of this patent
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Abstract
The present invention relates to polypeptide variants of porcine trypsin, to nucleic acid molecules encoding these variants, and to host cells comprising such nucleic acid molecules. It also relates to the use of these variants in methods for producing insulin. The invention further relates to the use of these variants as medicaments, as food ingredients, or as feed ingredients and to the use of these variants within a process of manufacturing a food ingredient or a feed ingredient.
Claims20
A method for the production of human insulin, an insulin analogue or a derivative of insulin, the method comprising using a variant of porcine trypsin, wherein the variant of porcine trypsin comprises:
- an amino acid sequence that has at least 91% sequence identity to SEQ ID NO:
- 1,
- wherein said amino acid sequence differs from SEQ ID NO:
- 1 at least by one or more amino acid substitutions at one or more positions corresponding to F24, S44, D56, G78, Y131, S172 and W193 of native porcine trypsin according to SEQ ID NO:
- 1,
- with the proviso that said amino acid sequence is not native porcine trypsin according to SEQ ID NO:
- 1;
- and
- with the proviso that said amino acid sequence is not porcine variant trypsin S172A according to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.