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US 11,952,598 B2Granted
Engineered glucosyltransferases
AssigneeNutrition & Biosciences USA 4, Inc.
InventorsYougen Li, Mark S. Payne, Jared B. Parker, Slavko Kralj, Veli Alkan, Richard R. Bott, Robert Dicosimo, Qiong Cheng, Ellen D. Semke, Susan Marie Hennessey
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Abstract
Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
Claims20
A non-native glucosyltransferase comprising an amino acid substitution at a position corresponding with amino acid residue Asp-948 of SEQ ID NO:62,
- wherein the non-native glucosyltransferase synthesizes alpha-glucan comprising 1,3-linkages, and
- wherein the non-native glucosyltransferase has:
- (i) an alpha-glucan yield that is higher than the alpha-glucan yield of a second glucosyltransferase that only differs from the non-native glucosyltransferase at the substitution position, and/or
- (ii) a leucrose yield that is lower than the leucrose yield of the second glucosyltransferase,
- wherein the non-native glucosyltransferase comprises a catalytic domain that is at least about 90% identical to residues 55-960 of SEQ ID NO:4, residues 54-957 of SEQ ID NO:65, residues 55-960 of SEQ ID NO:30, residues 55-960 of SEQ ID NO:28, or residues 55-960 of SEQ ID NO:20.
2 more elements
Dependencies are inferred from the claim text.