Method for producing platelets
AssigneesKyoto University, Megakaryon Corporation
InventorsKoji Eto, Sou Nakamura, Yukitaka Ito
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Abstract
Healthy functional platelets are mass produced. A method for producing platelets, comprising: (1) a culture step of culturing megakaryocytes in a platelet producing medium in the presence of mechanical stress and platelet production promoting factors including MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, and (2) a harvest step of harvesting the platelets obtained by the culture step; wherein the culture step comprises: (a) a step of promoting a release of the platelet production promoting factors from megakaryocytes by mechanical stress; and/or (b) a step of externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2.
Claims8
A method for producing platelets, comprising the steps of:
- (1) culturing megakaryocytes in a platelet producing medium, and
- (2) harvesting platelets obtained by the culturing;
- wherein the step of culturing comprises:
- (a) promoting a release of one or more platelet production promoting factor(s), wherein the factor(s) comprise MIF, NRDc, IGFBP2, TSP-1, PAI-1, or CCL5, from megakaryocytes by applying mechanical stress including turbulence by vortex;
- and/or
- (b) externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2,
- wherein the step of culturing comprises step (b) and wherein step (b) further comprises externally adding at least one platelet production promoting factor(s) comprising TSP-1, PAI-1, or CCL5.
Dependencies are inferred from the claim text.